PT - JOURNAL ARTICLE AU - Rupak Doshi AU - Aaron P. McGrath AU - Miguel Piñeros AU - Paul Szewczyk AU - Denisse M. Garza AU - Leon V. Kochian AU - Geoffrey Chang TI - Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from <em>Sorghum bicolor</em> AID - 10.1101/182964 DP - 2017 Jan 01 TA - bioRxiv PG - 182964 4099 - http://biorxiv.org/content/early/2017/08/31/182964.short 4100 - http://biorxiv.org/content/early/2017/08/31/182964.full AB - About 50% of the world’s arable land is strongly acidic (soil pH &lt; 5). The low pH of these soils solubilizes root-toxic ionic aluminium (Al3+) species from clay minerals, driving the evolution of various counteractive adaptations in cultivated crops. The food crop Sorghum bicolor, for example, upregulates the membrane-embedded transporter protein SbMATE in its roots. SbMATE mediates efflux of the anionic form of the organic acid, citrate, into the soil rhizosphere, chelating Al3+ ions and thereby imparting Al-resistance based on excluding Al+3 from the growing root tip. Here, we use electrophysiological, radiolabeled, and fluorescence-based transport assays in two heterologous expression systems to establish a broad substrate recognition profile of SbMATE, showing the transport of 14C- citrate anion, as well as the organic monovalent cation, ethidium, but not the divalent ethidium-derivative, propidium. The transport cycle is proton and/or sodium-driven, and shares certain molecular mechanisms with bacterial MATE-family transporters. We further complement our transport assays by directly measuring substrate binding to detergent-purified SbMATE protein. Finally, we use the functionally-folded, purified membrane protein as an antigen to discover high-affinity, native conformation-binding and transport function-altering nanobodies using an animal-free, mRNA/cDNA display technology. Our results demonstrate the utility of using Pichia pastoris as an efficient eukaryotic host to express large quantities of functional plant transporter proteins for in vitro characterization. The nanobody discovery approach is applicable to other low immunogenic plant proteins.