PT - JOURNAL ARTICLE AU - Sarah Robinson AU - Michal Huflejt AU - Pierre Barbier de Reuille AU - Siobhan A. Braybrook AU - Martine Schorderet AU - Didier Reinhardt AU - Cris Kuhlemeier TI - An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution AID - 10.1101/183533 DP - 2017 Jan 01 TA - bioRxiv PG - 183533 4099 - http://biorxiv.org/content/early/2017/09/01/183533.short 4100 - http://biorxiv.org/content/early/2017/09/01/183533.full AB - How complex developmental-genetic networks are translated into organs with specific 3D shapes remains an open question. This question is particularly challenging because the elaboration of specific shapes is in essence a question of mechanics. In plants, this means how the genetic circuitry affects the cell wall. The mechanical properties of the wall and their spatial variation are the key factors controlling morphogenesis in plants. However, these properties are difficult to measure and investigating their relation to genetic regulation is particularly challenging. To measure spatial variation of mechanical properties, one must determine the deformation of a tissue in response to a known force with cellular resolution. Here we present an automated confocal micro-extensometer (ACME), which greatly expands the scope of existing methods for measuring mechanical properties. Unlike classical extensometers, ACME is mounted on a confocal microscope and utilizes confocal images to compute the deformation of the tissue directly from biological markers, thus providing cellular scale information and improved accuracy. ACME is suitable for measuring the mechanical responses in live tissue. As a proof of concept we demonstrate that the plant hormone gibberellic acid induces a spatial gradient in mechanical properties along the length of the Arabidopsis hypocotyl.Stressis the force acting on the material per unit area.Strainthe relative increase in length of the material, and can be expressed as a percentage change in length.Mechanical propertiesdescribe the stress-strain relationship for a material. If we apply the same force to a material that is twice as thick/stiff? it will deform half as much, if the material is otherwise the same.Elasticelastic materials deform instantly and reversibly.Creepa time-dependent irreversible strain that occurs when a constant force is applied and maintained. Creep is measured using creep tests. A force is applied and maintained for a period of time. The force is removed to reveal the reversible and irreversible deformation.