PT - JOURNAL ARTICLE AU - Chaitali Khan AU - Sonia Muliyil AU - Champakali Ayyub AU - B J Rao TI - <em>dmrad51/spnA</em> mutant exhibit defects during somatic stages of developmental and show enhanced genomic damage, cell death and low temperature sensitivity AID - 10.1101/180737 DP - 2017 Jan 01 TA - bioRxiv PG - 180737 4099 - http://biorxiv.org/content/early/2017/09/06/180737.short 4100 - http://biorxiv.org/content/early/2017/09/06/180737.full AB - Homologous Recombination (HR) is one of the key pathways to repair Double Strand Breaks (DSBs). Rad51 serves an important function of catalysing strand exchange between two homologous chromosomes in the HR pathway. In higher organisms, Rad51 function is indispensable with its absence leading to early embryonic lethality, thus precluding any mechanistic probing of the system. In contrast, absence of Drosophila rad51 (Dmrad51/spnA) has been associated with defects in female germline causing ventralization of the egg, without any reported detrimental consequences to Drosophila somatic tissues. In this study, we have performed a systematic analysis of somatic development of dmrad51 null mutant flies by using genetic complementation between multiple dmrad51 alleles. Our current study, for the first time, uncovers the requirement of Dmrad51 in somatic tissue maintenance at both larval and pupal stages. Also, we show that dmrad51 mutant exhibit patterning defects in abdominal cuticle in the stripes and bristles, while there appears to be only subtle defects in the adult wing and eye. Interestingly, dmrad51 null mutant and other alleles show discernible phenotype of low temperature sensitivity, suggesting a role for Dmrad51 in temperature sensitive cellular processes, which thus presents an elegant system for probing temperature sensitive cellular/tissue responses that ensue when a mutation leads to the loss of protein expression (null mutant) rather than its altered protein structure. In summary, our study describes the role of Dmrad51 during somatic stages of development and provides a viable model system to study Rad51 function in a cellular process.