RT Journal Article
SR Electronic
T1 A rainfall-manipulation experiment with 517 <em>Arabidopsis thaliana</em> accessions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 186767
DO 10.1101/186767
A1 Moises Exposito-Alonso
A1 Rocío Gómez Rodríguez
A1 Cristina Barragán
A1 Giovanna Capovilla
A1 Eunyoung Chae
A1 Jane Devos
A1 Ezgi S. Dogan
A1 Claudia Friedemann
A1 Caspar Gross
A1 Patricia Lang
A1 Derek Lundberg
A1 Vera Middendorf
A1 Jorge Kageyama
A1 Talia Karasov
A1 Sonja Kersten
A1 Sebastian Petersen
A1 Leily Rabbani
A1 Julian Regalado
A1 Lukas Reinelt
A1 Beth Rowan
A1 Danelle K. Seymour
A1 Efthymia Symeonidi
A1 Rebecca Schwab
A1 Diep Thi Ngoc Tran
A1 Kavita Venkataramani
A1 Anna-Lena Van de Weyer
A1 François Vasseur
A1 George Wang
A1 Ronja Wedegärtner
A1 Frank Weiss
A1 Rui Wu
A1 Wanyan Xi
A1 Maricris Zaidem
A1 Wangsheng Zhu
A1 Fernando García-Arenal
A1 Hernán A. Burbano
A1 Oliver Bossdorf
A1 Detlef Weigel
YR 2017
UL http://biorxiv.org/content/early/2017/09/10/186767.abstract
AB The gold standard for studying natural selection is to quantify lifetime fitness in individuals from natural populations that have been grown together under different field conditions. This has been widely done in ecology to measure phenotypic selection in nature for a wide range of organisms — an evolutionary force that seems to be most determined by local precipitation patterns. Studies that include whole-genome data would enable the translation of coefficients of selection to the genetic level, but such studies are still scarce, even though this type of genetic knowledge will be critical to predict the effect of climate change in natural populations. Here we present such an experiment including rainfall-manipulation with the plant A rabidopsis thaliana. The experiment was carried out in a Mediterranean and a Central European field station with rainout shelters to simulate a high and low rainfall treatment within each location. For each treatment combination, we planted 7 pots with one individual and 5 pots with 30 counted seeds of 517 whole-genome sequenced natural accessions covering the global species distribution. Survival, germination, flowering time, and final seed output were measured for ca. 25,000 pots, which contained ca. 14,500 individual plants and over 310,00 plants growing in small populations. This high-throughput phenotyping was only possible thanks to image analysis techniques using custom-made scripts. To make the data and processing code available, we created an R package “dryAR” (http://github.com/MoisesExpositoAlonso/dryAR).