TY - JOUR T1 - An <em>ex vivo</em> cystic fibrosis model recapitulates key clinical aspects of chronic <em>Staphylococcus aureus</em> infection JF - bioRxiv DO - 10.1101/604363 SP - 604363 AU - Esther Sweeney AU - Marwa M. Hassan AU - Niamh E. Harrington AU - Alan R. Smyth AU - Matthew N. Hurley AU - María Ángeles Tormo-Mas AU - Freya Harrison Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/04/09/604363.abstract N2 - Staphylococcus aureus is one of the most prevalent organisms isolated from the airways of people with cystic fibrosis (CF), predominantly early in life. Yet its role in the pathology of lung disease is poorly understood. Clinical studies are limited in scope by age and health of participants and in vitro studies are not always able to accurately recapitulate chronic disease characteristics such as the development of small colony variants. Further, animal models also do not fully represent features of clinical disease: in particular, mice are not readily colonized by S. aureus and when infection is established it leads to the formation of abscesses, a phenomenon almost never observed in the human CF lung. Here, we present details of the development of an existing ex vivo pig lung model of CF infection to investigate the growth of S. aureus. We show that S. aureus is able to establish infection and demonstrates clinically significant characteristics including small colony variant phenotype, increased antibiotic tolerance and preferential localisation in mucus. Tissue invasion and the formation of abscesses were not observed, in line with clinical data. ER -