PT  - JOURNAL ARTICLE
AU  - Philipp Rosendahl
AU  - Katarzyna Plak
AU  - Angela Jacobi
AU  - Martin Kraeter
AU  - Nicole Toepfner
AU  - Oliver Otto
AU  - Christoph Herold
AU  - Maria Winzi
AU  - Maik Herbig
AU  - Yan Ge
AU  - Salvatore Girardo
AU  - Katrin Wagner
AU  - Buzz Baum
AU  - Jochen Guck
TI  - Real-time fluorescence and deformability cytometry — flow cytometry goes mechanics
AID  - 10.1101/187435
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 187435
4099  - http://biorxiv.org/content/early/2017/09/11/187435.short
4100  - http://biorxiv.org/content/early/2017/09/11/187435.full
AB  - Cell mechanical characterization has recently approached the throughput of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we combine real-time 1D-imaging fluorescence and deformability cytometry (RT-FDC) to merge the two worlds in one instrument — opening many new research avenues. We demonstrate its utility using sub-cellular fluorescence localization to identify mitotic cells and test for their mechanical changes in an RNAi screen.