RT Journal Article
SR Electronic
T1 Single-cell transcriptomic profiling of progenitors of the oligodendrocyte lineage reveals transcriptional convergence during development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 186445
DO 10.1101/186445
A1 Sueli Marques
A1 Darya Vanichkina
A1 David van Bruggen
A1 Elisa M. Floriddia
A1 Hermany Munguba
A1 Leif Väremo
A1 Stefania Giacomello
A1 Ana Mendanha Falcão
A1 Mandy Meijer
A1 S Samudyata
A1 Simone Codeluppi
A1 Åsa K. Björklund
A1 Sten Linnarsson
A1 Jens Hjerling-Leffler
A1 Ryan J. Taft
A1 Gonçalo Castelo-Branco
YR 2017
UL http://biorxiv.org/content/early/2017/09/17/186445.abstract
AB Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different transcriptional oligodendrocyte (OL) states at adult stages. Here we present a bulk and single-cell transcriptomics resource providing insights on how transitions between these states occur. We show that E13.5 Pdgfra+ populations are not OPCs, exhibiting instead hallmarks of neural progenitors. A subset of these progenitors, which we refer as pre-OPCs, rewires their transcriptional landscape, converging into indistinguishable OPC states at E17.5 and post-natal stages. P7 brain and spinal cord OPCs present similar transcriptional profiles at the single-cell level, indicating that OPC states are not region-specific. Postnatal OPC progeny of E13.5 Pdgfra+ have electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves. In addition, lineage tracing indicates that a subset of E13.5 Pdgfra+ cells also originate cells of the pericyte lineage. In summary, our results indicate that embryonic Pdgfra+ cells are diverse and give rise at post-natal stages to distinct cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.