PT  - JOURNAL ARTICLE
AU  - Francisco Acosta-Reyes
AU  - Ritam Neupane
AU  - Joachim Frank
AU  - Israel S. Fernández
TI  - The Israeli Acute Paralysis Virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs
AID  - 10.1101/606236
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 606236
4099  - http://biorxiv.org/content/early/2019/04/11/606236.short
4100  - http://biorxiv.org/content/early/2019/04/11/606236.full
AB  - The Colony Collapse Disorder or CCD is a multi-faceted syndrome decimating bee populations worldwide[1]. A group of viruses of the widely distributed Dicistroviridae family have been identified as a causing agent of CCD[2]. This family of viruses employ non-coding RNA sequences, called Internal Ribosomal Entry Site (IRES), to precisely exploit the host machinery for protein production. Using single-particle cryo-electron microscopy (cryo-EM) we have characterized at high resolution how the IRES of the intergenic region of the Israeli Acute Paralysis Virus (IAPV) captures and redirects translating ribosomes towards viral messengers. Through a series of six structures at nominal resolutions close to 3Å, we could reconstruct the trajectory of IAPV-IRES from an early small subunit recruitment to a final post-translocated state in the ribosome. An early commitment of IRES/ribosome complexes for global pre-translocation mimicry explains the high efficiency observed for this IRES. The presented structures will help guide on-going efforts directed towards fighting CCD through RNA-interference technology [3].