RT Journal Article
SR Electronic
T1 Eye tracking-based estimation and compensation of chromatic offsets for multi-wavelength retinal microstimulation with foveal cone precision
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 599464
DO 10.1101/599464
A1 Niklas Domdei
A1 Michael Linden
A1 Jenny L. Reiniger
A1 Frank G. Holz
A1 Wolf M. Harmening
YR 2019
UL http://biorxiv.org/content/early/2019/04/11/599464.abstract
AB Multi-wavelength ophthalmic imaging and stimulation of photoreceptor cells requires consideration of chromatic dispersion of the eye, manifesting in longitudinal and transverse chromatic aberrations. Current image-based techniques to measure and correct transverse chromatic aberration (TCA) and the resulting transverse chromatic offset (TCO) in an adaptive optics retinal imaging system are precise, but lack compensation of small but significant shifts in eye position occurring during in vivo testing. Here we present a method that requires only a single measurement of TCO during controlled movements of the eye to map retinal chromatic image shifts to the image space of a pupil camera. After such calibration, TCO can be compensated by continuously monitoring eye position during experimentation and by interpolating correction vectors from a linear fit to the calibration data. The average change rate of TCO per head shift and the correlation between Kappa and the individual foveal TCA are close to the expectations based on a chromatic eye model. Our solution enables continuous correction of TCO with high spatial precision and avoids high light intensities required for re-measuring TCO after eye position changes, which is necessary for foveal cone-targeted psychophysical experimentation.