RT Journal Article
SR Electronic
T1 Biogenesis of a mitochondrial DNA inheritance machinery in the mitochondrial outer membrane
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 190751
DO 10.1101/190751
A1 Sandro Käser
A1 Mathilde Willemin
A1 Felix Schnarwiler
A1 Bernd Schimanski
A1 Daniel Poveda-Huertes
A1 Silke Oeljeklaus
A1 Bettina Warscheid
A1 Chris Meisinger
A1 André Schneider
YR 2017
UL http://biorxiv.org/content/early/2017/09/19/190751.abstract
AB Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoan Trypanosoma brucei has a single mitochondrion with a single-unit genome that is physically connected across the mitochondrial membranes to the basal body of the flagellum. This connection, termed tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins - TAC60, TAC42 and TAC40 - which are essential subunits of the TAC. TAC60 contains separable mitochondrial import and TAC-sorting signals and its biogenesis depends on the main outer membrane protein translocase. TAC40 is a member of the mitochondrial porin family, whereas TAC42 represents a novel class of mitochondrial outer membrane β-barrel proteins. Consequently TAC40 and TAC42 contain C-terminal β-signals. Thus in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.