RT Journal Article
SR Electronic
T1 Urinary candidate biomarkers in an experimental autoimmune myocarditis rat model
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 191379
DO 10.1101/191379
A1 Mindi Zhao
A1 Jiangqiang Wu
A1 Xundou Li
A1 Youhe Gao
YR 2017
UL http://biorxiv.org/content/early/2017/09/20/191379.abstract
AB Urine is a better source than plasma for biomarker studies, as it can accumulate all changes in the body. Various candidate urinary biomarkers of physiological conditions, kidney disease and even brain dysfunction, have been detected in urine; however, urine has rarely been used to reflect cardiac diseases. As the clinical presentations of myocarditis are heterogeneous, reliable and sensitive diagnostic biomarkers of myocarditis are very important. In this study, candidate urinary biomarkers in the myosin-induced autoimmune myocarditis rat models were characterized using the isobaric tandem mass tag labeling approach coupled with high-resolution mass spectrometry. Compared with controls, forty-six urinary proteins were significantly changed in the myocarditis rats; among them, ten had previously been associated with myocarditis, twelve corresponding gene products have been annotated as mainly cardiovascular network genes by the Ingenuity Pathway Analysis, and four urinary proteins were validated by western blot.