RT Journal Article
SR Electronic
T1 Single Cell RNA Sequencing of stem cell-derived retinal ganglion cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 191395
DO 10.1101/191395
A1 Maciej Daniszewski
A1 Anne Senabouth
A1 Quan Nguyen
A1 Duncan E. Crombie
A1 Samuel W. Lukowski
A1 Tejal Kulkarni
A1 Donald J Zack
A1 Alice Pébay
A1 Joseph E. Powell
A1 Alex W. Hewitt
YR 2017
UL http://biorxiv.org/content/early/2017/09/22/191395.abstract
AB We used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and subsequently prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells upregulated genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature phenotype.