PT  - JOURNAL ARTICLE
AU  - Jeffrey N. Stirman
AU  - Ikuko T. Smith
AU  - Michael W. Kudenov
AU  - Spencer L. Smith
TI  - Wide field-of-view, multi-region two-photon imaging of neuronal activity <em>in vivo</em>
AID  - 10.1101/011320
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 011320
4099  - http://biorxiv.org/content/early/2015/07/08/011320.short
4100  - http://biorxiv.org/content/early/2015/07/08/011320.full
AB  - We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.