RT Journal Article
SR Electronic
T1 Development of an Intrinsic Skin Sensor for Blood Glucose Level with CRISPR-mediated Genome Editing in Epidermal Stem Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 195529
DO 10.1101/195529
A1 Yue, Jiping
A1 Li, Yuanyuan
A1 Gou, Xuewen
A1 Wu, Xiaoyang
YR 2017
UL http://biorxiv.org/content/early/2017/09/28/195529.abstract
AB Biointegrated sensors can address various challenges in medicine by transmitting a wide variety of biological signals. A tempting possibility that has not been explored before is whether we can take advantage of genome editing technology to transform a small portion of endogenous tissue into an intrinsic and long-lasting sensor of physiological signals. The human skin and the epidermal stem cells have several unique advantages, making them particularly suitable for genetic engineering and applications in vivo. In this report, we took advantage of a novel platform for manipulation and transplantation of epidermal stem cells, and presented the key evidence that genome-edited skin stem cells can be exploited for continuous monitoring of blood glucose level in vivo. Additionally, by advanced design of genome editing, we developed an autologous skin graft that can sense glucose level and deliver therapeutic proteins for diabetes treatment. Our results revealed the clinical potential for skin somatic gene therapy.