RT Journal Article
SR Electronic
T1 CRISPR-Based DNA Imaging in Living Cells Reveals Cell Cycle-Dependent Chromosome Dynamics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 195966
DO 10.1101/195966
A1 Hanhui Ma
A1 Li-Chun Tu
A1 Ardalan Naseri
A1 Yu-Chieh Chung
A1 David Grunwald
A1 Shaojie Zhang
A1 Thoru Pederson
YR 2017
UL http://biorxiv.org/content/early/2017/09/29/195966.abstract
AB In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics in interphase are less understood. We developed a sensitive, multicolor system, CRISPR-Sirius, allowing the real-time tracking of the dynamics of chromosomal loci. We tracked loci kilobases to megabases apart and found significant variation in the inter-locus distances of each pair, indicating differing degrees of DNA contortion. We resolved two distinct modes of dynamics of loci: saltatory local movements as well as translational movements of the domain. The magnitude of both of these modes of movements increased from early to late G1, whereas the translational movements were reduced in early S. The local fluctuations decreased slightly in early S and more markedly in mid-late S. These newly observed movements and their cell cycle-dependence are indicative of a hitherto unrecognized compaction-relaxation dynamic of the chromosomal fiber operating concurrently with changes in the extent of observed genomic domain movements.IN BRIEF Distinct chromosome folding and dynamics during cell cycle progression were dissected by CRISPR-Sirius DNA imaging in living cells.HIGHLIGHTSCRISPR-Sirius allows tracking of pairs of chromosomal loci having kilobase to megabase inter-locus distancesPair-wise tracking of loci allows measurement of both local and domain dynamicsChromosomal fiber relaxation is positively correlated with local dynamicsGenomic region size contributes to local and domain movementsDistinct chromosome dynamics were uncovered during cell cycle progression in interphase