PT  - JOURNAL ARTICLE
AU  - Andrew E S Barentine
AU  - Yu Lin
AU  - Miao Liu
AU  - Phylicia Kidd
AU  - Leonhard Balduf
AU  - Michael R Grace
AU  - Siyuan Wang
AU  - Joerg Bewersdorf
AU  - David Baddeley
TI  - 3D Multicolor Nanoscopy at 10,000 Cells a Day
AID  - 10.1101/606954
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 606954
4099  - http://biorxiv.org/content/early/2019/04/12/606954.short
4100  - http://biorxiv.org/content/early/2019/04/12/606954.full
AB  - Diffraction-unlimited single-molecule switching (SMS) nanoscopy techniques like STORM /(F)PALM enable three-dimensional (3D) fluorescence imaging at 20-80 nm resolution and are invaluable to investigate sub-cellular organization. They suffer, however, from low throughput, limiting the output of a days worth of imaging to typically a few tens of mammalian cells. Here we develop an SMS imaging platform that combines high-speed 3D single-molecule data acquisition with an automated, fully integrated, high-volume data processing pipeline. We demonstrate 2-color 3D super-resolution imaging of over 10,000 mammalian cell nuclei in about 26 hours, connecting the traditionally low-throughput super-resolution community to the world of omics approaches.