PT  - JOURNAL ARTICLE
AU  - Lena Gamboa
AU  - Erick V. Phung
AU  - Haoxin Li
AU  - Jared P. Meyers
AU  - Gabriel A. Kwong
TI  - Heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation
AID  - 10.1101/606723
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 606723
4099  - http://biorxiv.org/content/early/2019/04/12/606723.short
4100  - http://biorxiv.org/content/early/2019/04/12/606723.full
AB  - Emerging CRISPR technologies are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially-defined, noninvasive modalities to direct their function limit their potential as biological tools and pose a major challenge for clinical translation. Here we confer remote control of CRISPR-dCas9 activity using thermal gene switches, enabling the dynamic regulation of gene expression using short pulses of heat to modulate transcriptional commands.