@article {Lewis185694, author = {Samuel H. Lewis and Kaycee A. Quarles and Yujing Yang and Melanie Tanguy and Lise Fr{\'e}zal and Stephen A. Smith and Prashant P. Sharma and Richard Cordaux and Cl{\'e}ment Gilbert and Isabelle Giraud and David H. Collins and Phillip D. Zamore and Eric A. Miska and Peter Sarkies and Francis M. Jiggins}, title = {Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements}, elocation-id = {185694}, year = {2017}, doi = {10.1101/185694}, publisher = {Cold Spring Harbor Laboratory}, abstract = {In animals, small RNA molecules termed PIWI-interacting RNAs (piRNAs) silence transposable elements (TEs), protecting the germline from genomic instability and mutation. piRNAs have been detected in the soma in a few animals, but these are believed to be specific adaptations of individual species. Here, we report that somatic piRNAs were likely present in the ancestral arthropod more than 500 million years ago. Analysis of 20 species across the arthropod phylum suggests that somatic piRNAs targeting TEs and mRNAs are common among arthropods. The presence of an RNA-dependent RNA polymerase in chelicerates (horseshoe crabs, spiders, scorpions) suggests that arthropods originally used a plant-like RNA interference mechanism to silence TEs. Our results call into question the view that the ancestral role of the piRNA pathway was to protect the germline and demonstrate that small RNA silencing pathways have been repurposed for both somatic and germline functions throughout arthropod evolution.}, URL = {https://www.biorxiv.org/content/early/2017/10/04/185694}, eprint = {https://www.biorxiv.org/content/early/2017/10/04/185694.full.pdf}, journal = {bioRxiv} }