PT - JOURNAL ARTICLE AU - Samuel H. Lewis AU - Kaycee A. Quarles AU - Yujing Yang AU - Melanie Tanguy AU - Lise Frézal AU - Stephen A. Smith AU - Prashant P. Sharma AU - Richard Cordaux AU - Clément Gilbert AU - Isabelle Giraud AU - David H. Collins AU - Phillip D. Zamore AU - Eric A. Miska AU - Peter Sarkies AU - Francis M. Jiggins TI - Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements AID - 10.1101/185694 DP - 2017 Jan 01 TA - bioRxiv PG - 185694 4099 - http://biorxiv.org/content/early/2017/10/04/185694.short 4100 - http://biorxiv.org/content/early/2017/10/04/185694.full AB - In animals, small RNA molecules termed PIWI-interacting RNAs (piRNAs) silence transposable elements (TEs), protecting the germline from genomic instability and mutation. piRNAs have been detected in the soma in a few animals, but these are believed to be specific adaptations of individual species. Here, we report that somatic piRNAs were likely present in the ancestral arthropod more than 500 million years ago. Analysis of 20 species across the arthropod phylum suggests that somatic piRNAs targeting TEs and mRNAs are common among arthropods. The presence of an RNA-dependent RNA polymerase in chelicerates (horseshoe crabs, spiders, scorpions) suggests that arthropods originally used a plant-like RNA interference mechanism to silence TEs. Our results call into question the view that the ancestral role of the piRNA pathway was to protect the germline and demonstrate that small RNA silencing pathways have been repurposed for both somatic and germline functions throughout arthropod evolution.