TY - JOUR T1 - The Elastic Network Contact Model applied to RNA: enhanced accuracy for conformational space prediction JF - bioRxiv DO - 10.1101/198531 SP - 198531 AU - Olivier Mailhot AU - Vincent Frappier AU - François Major AU - Rafael Najmanovich Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/10/04/198531.abstract N2 - Motivation The use of Normal Mode Analysis (NMA) methods to study both protein and nucleic acid dynamics is well established. However, the most widely used coarse-grained methods are based on backbone geometry alone and do not take into account the chemical nature of the residues. Elastic Network Contact Model (ENCoM) is a coarse-grained NMA method that includes a pairwise atom-type non-bonded interaction term, which makes it sensitive to the sequence of the studied molecule. We adapted ENCoM to simulate the dynamics of ribonucleic acid (RNA) molecules.Results ENCoM outperforms the most commonly used coarse-grained model on RNA, Anisotropic Network Model (ANM), in the prediction of b-factors, in the prediction of conformational change as measured by overlap (a measure of effective prediction of structural transitions) and in the prediction of structural variance from NMR ensembles. These benchmarks were derived from the set of all RNA structures available from the Protein Data Bank (PDB) and contain more total cases than previous studies applying NMA to RNA. We thus established ENCoM as an attractive tool for fast and accurate exploration of the conformational space of RNA molecules.Availability ENCoM is open source software available at https://github.com/NRGlab/ENCoM ER -