PT  - JOURNAL ARTICLE
AU  - Meytal Radzinski
AU  - Rosi Fasler
AU  - Ohad Yogev
AU  - William Breuer
AU  - Nadav Shai
AU  - Jenia Gutin
AU  - Tommer Ravid
AU  - Nir Friedman
AU  - Maya Schuldiner
AU  - Dana Reichmann
TI  - Temporal profiling of redox-dependent heterogeneity in single cells
AID  - 10.1101/198408
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 198408
4099  - http://biorxiv.org/content/early/2017/10/04/198408.short
4100  - http://biorxiv.org/content/early/2017/10/04/198408.full
AB  - Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe roGFP. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach we defined redox-dependent heterogeneity of yeast cells, and characterized growth, as well as proteomic and transcriptomic profiles of subpopulations of cells that differ in their redox status, but are similar in age. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis.