RT Journal Article
SR Electronic
T1 Neocortical microdissection at columnar and laminar resolution for molecular interrogation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 200931
DO 10.1101/200931
A1 Koen Kole
A1 Tansu Celikel
YR 2017
UL http://biorxiv.org/content/early/2017/10/10/200931.abstract
AB The heterogeneous organization of the mammalian neocortex poses a challenge to elucidate the molecular mechanisms underlying its physiological processes. Although high-throughput molecular methods are increasingly deployed in neuroscience, their anatomical specificity is often lacking. Here we introduce a targeted microdissection technique that enables extraction of high-quality RNA and proteins at high anatomical resolution from acutely prepared brain slices. We exemplify its utility by isolating single cortical columns and laminae from the mouse primary somatosensory (barrel) cortex. Tissues can be isolated from living slices in minutes, and the extracted RNA and protein are of sufficient quantity and quality to be used for RNA-sequencing and mass spectrometry. This technique will help to increase the anatomical specificity of molecular studies of the neocortex, and the brain in general as it is applicable to any brain structure that can be identified using optical landmarks in living slices.