RT Journal Article
SR Electronic
T1 Imaging Membrane Curvature inside a Model Immunological Synapse in RBL-2H3 Cells using TIRF Microscopy with Polarized Excitation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 608463
DO 10.1101/608463
A1 Rosa Machado
A1 Justin Bendesky
A1 Madison Brown
A1 Kathrin Spendier
A1 Guy M. Hagen
YR 2019
UL http://biorxiv.org/content/early/2019/04/13/608463.abstract
AB Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) coming into contact with a supported lipid bilayer, modeling an immunological synapse. These studies help correlate the dynamics of cell surface molecules with the mechanical properties of the plasma membrane during synapse formation.