RT Journal Article SR Electronic T1 Circlator: automated circularization of genome assemblies using long sequencing reads JF bioRxiv FD Cold Spring Harbor Laboratory SP 023408 DO 10.1101/023408 A1 Martin Hunt A1 Nishadi De Silva A1 Thomas D Otto A1 Julian Parkhill A1 Jacqueline A Keane A1 Simon R Harris YR 2015 UL http://biorxiv.org/content/early/2015/07/30/023408.abstract AB The assembly of DNA sequence data into finished genomes is undergoing a renais-sance thanks to emerging technologies producing reads of tens of kilobases. Assembling complete bacterial and small eukaryotic genomes is now possible, but the final step of circularizing sequences remains unsolved. Here we present Circlator, the first tool to automate assembly circularization and produce accurate linear rep-resentations of circular sequences. Using Pacific Biosciences and Oxford Nanopore data, Circlator correctly circularized 26 of 27 circularizable sequences, comprising 11 chromosomes and 12 plasmids from bacteria, the apicoplast and mitochondrion of Plasmodium falciparum and a human mitochondrion. Circlator is available at http://sanger-pathogens.github.io/circlator/.NCTCNational Collection of Type CulturesONTOxford Nanopore TechnologiesPacBioPacific Biosciences