@article {Rinc{\'o}n204875, author = {Esther Rinc{\'o}n and Briana Rocha-Gregg and Sean R. Collins}, title = {A map of gene expression in neutrophil-like cell lines}, elocation-id = {204875}, year = {2017}, doi = {10.1101/204875}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Background Human neutrophils are central players in innate immunity, a major component of inflammatory responses, and a leading model for cell motility and chemotaxis. However, primary neutrophils are remarkably short-lived, limiting their experimental usefulness in the laboratory. Thus, human myeloid cell lines have been established and characterized for their ability to undergo neutrophil-like differentiation in vitro. The HL-60 cell line and its PLB-985 sub-line are commonly used as a model for human neutrophil behavior, but how closely gene expression in differentiated cells resembles that of primary neutrophils has remained unclear.Results In the present study, we compared the effectiveness of differentiation protocols and used RNA sequencing (RNA-seq) to compare the transcriptomes of HL-60 and PLB-985 cells with published data for human and mouse primary neutrophils. Among commonly used differentiation protocols for neutrophil like cell lines, addition of dimethyl sulfoxide (DMSO) gave the best combination of cell viability and expression of markers for differentiation. However, combining DMSO with the serum-free-supplement Nutridoma resulted in an increased chemotactic response and cell surface expression of the neutrophil markers FPR1 and CD11b without a cost in viability. RNA-seq analysis of HL-60 and PLB-985 cells before and after differentiation showed that differentiation broadly increases the similarity in gene expression between the cell lines and primary neutrophils. Furthermore, the gene expression pattern of the differentiated cell lines correlated slightly better with that of human neutrophils than the mouse neutrophil pattern did. Finally, we created a publicly available gene expression database that is searchable by gene name and by protein domain content, where users can compare gene expression in HL-60, PLB-985 and primary human and mouse neutrophils.Conclusions Our study verifies that a DMSO-based differentiation protocol for HL-60 and PLB-985 cell lines gives superior differentiation and cell viability relative to other common protocols, and indicates that addition of Nutridoma may be preferable for studies of chemotaxis. Our neutrophil gene expression database will be a valuable tool to identify similarities and differences in gene expression between the cell lines and primary neutrophils, to compare expression levels for genes of interest, and to improve the design of tools for genetic perturbations.(RNA-seq)RNA sequencing(DMSO)Dimethyl sulfoxide(ATRA)All-trans retinoic acid(dbcAMP)Dibutyryl cyclic adenosine monophosphate(fMLF)N-formyl-methionyl-leucyl phenylalanine(DMF)N,N-Dimethyl formamide(FPR1)Formyl Peptide Receptor 1(FLPEP)Fluorescent N-formyl-methionyl-leucyl phenylalanine (fMLF) peptide ligand(CD11b)Integrin Alpha M, ITGAM(CA)Caffeic acid(MFI)Mean fluorescence intensity(FBS)Fetal bovine serum(NBT)Nitro Blue tetrazolium(G-CSF)Granulocyte colony-stimulating factor(GEF)Guanine nucleotide exchange factor(SNPs)Single-nucleotide polymorphisms(GATK)Genome Analysis Toolkit(NCBI)National Center for Biotechnology Information(FBS)Fetal bovine serum(P/S)Streptomycin and penicillin(PBS)Phosphate-buffered saline(FACS)Fluorescence-activated cell sorting(PMA)Phorbol 12-myristate 13-acetate}, URL = {https://www.biorxiv.org/content/early/2017/10/17/204875}, eprint = {https://www.biorxiv.org/content/early/2017/10/17/204875.full.pdf}, journal = {bioRxiv} }