TY - JOUR T1 - An Oxidative Pathway of Deoxyribose Catabolism JF - bioRxiv DO - 10.1101/205583 SP - 205583 AU - Jayashree Ray AU - Morgan N. Price AU - Anthony T. Iavarone AU - Hans K. Carlson AU - Elizabeth M. Ryan AU - Rex R. Malmstrom AU - Adam P. Arkin AU - Adam M. Deutschbauer Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/10/18/205583.abstract N2 - High-throughput genetics is a powerful approach to identify new genes involved in bacterial carbon catabolism. Here, we used genome-wide fitness assays to identify a novel pathway for 2-deoxy-D-ribose catabolism in Pseudomonas simiae WCS417. The genes that were important for deoxyribose utilization but not in other conditions included two putative dehydrogenases, a lactonase, a β-keto acid cleavage enzyme, and a glycerate kinase. We propose that deoxyribose is oxidized twice to 2-deoxy-3-keto-D-ribonoate (a β-keto acid) before cleavage to D-glycerate, which is phosphorylated and enters lower glycolysis. We purified the two dehydrogenases and reconstituted the enzymatic pathway for the conversion of deoxyribose to 2-deoxy-3-keto-D-ribonoate in vitro. ER -