RT Journal Article
SR Electronic
T1 An Oxidative Pathway of Deoxyribose Catabolism
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 205583
DO 10.1101/205583
A1 Jayashree Ray
A1 Morgan N. Price
A1 Anthony T. Iavarone
A1 Hans K. Carlson
A1 Elizabeth M. Ryan
A1 Rex R. Malmstrom
A1 Adam P. Arkin
A1 Adam M. Deutschbauer
YR 2017
UL http://biorxiv.org/content/early/2017/10/18/205583.abstract
AB High-throughput genetics is a powerful approach to identify new genes involved in bacterial carbon catabolism. Here, we used genome-wide fitness assays to identify a novel pathway for 2-deoxy-D-ribose catabolism in Pseudomonas simiae WCS417. The genes that were important for deoxyribose utilization but not in other conditions included two putative dehydrogenases, a lactonase, a β-keto acid cleavage enzyme, and a glycerate kinase. We propose that deoxyribose is oxidized twice to 2-deoxy-3-keto-D-ribonoate (a β-keto acid) before cleavage to D-glycerate, which is phosphorylated and enters lower glycolysis. We purified the two dehydrogenases and reconstituted the enzymatic pathway for the conversion of deoxyribose to 2-deoxy-3-keto-D-ribonoate in vitro.