@article {Perraki205765, author = {Artemis Perraki and Julien Gronnier and Paul Gouguet and Marie Boudsocq and Anne-Flore Deroubaix and Vincent Simon and Sylvie German-Retana and Cyril Zipfel and Emmanuelle Bayer and S{\'e}bastien Mongrand and V{\'e}ronique Germain}, title = {The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection}, elocation-id = {205765}, year = {2017}, doi = {10.1101/205765}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3-s phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.}, URL = {https://www.biorxiv.org/content/early/2017/10/19/205765}, eprint = {https://www.biorxiv.org/content/early/2017/10/19/205765.full.pdf}, journal = {bioRxiv} }