RT Journal Article
SR Electronic
T1 The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 205765
DO 10.1101/205765
A1 Artemis Perraki
A1 Julien Gronnier
A1 Paul Gouguet
A1 Marie Boudsocq
A1 Anne-Flore Deroubaix
A1 Vincent Simon
A1 Sylvie German-Retana
A1 Cyril Zipfel
A1 Emmanuelle Bayer
A1 Sébastien Mongrand
A1 Véronique Germain
YR 2017
UL http://biorxiv.org/content/early/2017/10/19/205765.abstract
AB Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3-s phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.