RT Journal Article SR Electronic T1 Proteolytic control of centrosome activity by APC/C-Polo maintains oocyte fate in Drosophila JF bioRxiv FD Cold Spring Harbor Laboratory SP 202465 DO 10.1101/202465 A1 A. L. Braun A1 F. Meghini A1 G. Villa-Fombuena A1 M. Guermont A1 E. M. Fernandez A1 M. D. Martín-Bermudo A1 A. González-Reyes A1 Y. Kimata YR 2017 UL http://biorxiv.org/content/early/2017/10/22/202465.abstract AB In metazoans, the centrioles/centrosomes of the female germline are eliminated prior to the end of oogenesis. Although this phenomenon is important for reproduction, the function of the centrosomes during oogenesis and the mechanism controlling their stability is still unclear. Here we demonstrate that the multi-subunit ubiquitin ligase, the Anaphase Promoting Complex/Cyclosome (APC/C), ensures normal germline development by regulating the activity and stability of centrosomes during early Drosophila oogenesis. We show that perturbing APC/C activity by altering the activity of its co-factor Vihar/Ube2c causes ectopic microtubule-nucleation activity of the centrosomes, resulting in a failure of oocyte specification and decreased female fertility. Altering APC/C regulation induces precocious destruction of the key centrosome regulator, Polo Kinase, which leads to destabilization of the centrosomes and blocks their migration into the oocyte. Our study highlights the critical importance of the spatiotemporal regulation of centrosomes by the proteolytic mechanism during metazoan oocyte development.