RT Journal Article SR Electronic T1 Progress toward the identification and stacking of crucial domestication traits in pennycress JF bioRxiv FD Cold Spring Harbor Laboratory SP 609990 DO 10.1101/609990 A1 Ratan Chopra A1 Evan B. Johnson A1 Ryan Emenecker A1 Edgar B. Cahoon A1 Joe Lyons A1 Daniel J. Kliebenstein A1 Erin Daniels A1 Kevin M. Dorn A1 Maliheh Esfahanian A1 Nicole Folstad A1 Katherine Frels A1 Michaela McGinn A1 Matthew Ott A1 Cynthia Gallaher A1 Kayla Altendorf A1 Alexandra Berroyer A1 Baraem Ismail A1 James A. Anderson A1 Donald L. Wyse A1 Tim Umaslov A1 John C. Sedbrook A1 M. David Marks YR 2019 UL http://biorxiv.org/content/early/2019/04/16/609990.abstract AB The oilseed species Thlaspi arvense (pennycress) is being domesticated as a new crop that can provide both important ecosystem services and intensify farmland output. Through the use of high throughput sequencing and phenotyping, along with classical mutagenesis key traits needed for pennycress domestication have been identified. Domestication traits identified herein include reduced pod shatter, early maturity, reduced seed glucosinolate levels, and improved oil fatty acid content. By taking advantage of pennycress’ close genetic relationship with Arabidopsis thaliana, the causative mutations responsible for each of these traits have been identified. These mutations have been used to develop molecular markers to begin to stack the traits into individual lines.