RT Journal Article
SR Electronic
T1 An Alternative Framework for Fluorescence Correlation Spectroscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 426114
DO 10.1101/426114
A1 Sina Jazani
A1 Ioannis Sgouralis
A1 Omer M. Shafraz
A1 Marcia Levitus
A1 Sanjeevi Sivasankar
A1 Steve Pressé
YR 2019
UL http://biorxiv.org/content/early/2019/04/16/426114.abstract
AB Fluorescence correlation spectroscopy (FCS), is a flexible and widely used tool routinely exploited for in vivo and in vitro applications. While FCS provides estimates of dynamical quantities, such as diffusion coefficients, it demands high signal to noise ratios and long time traces, typically in the minute range. In principle, the same information can be extracted from µ-s long time traces; however, an appropriate analysis method is missing. To overcome these limitations, we adapt novel tools inspired by Bayesian non-parametrics, which starts from the direct analysis of the observed photon counts. With this approach, we are able to analyze time traces, which are too short to be analyzed by existing methods, including FCS. Our new analysis extends the capability of single molecule fluorescence confocal microscopy based approaches, to probe processes several orders of magnitude faster in time and permits a reduction of phototoxic effects on living samples induced by long periods of light exposure.