RT Journal Article
SR Electronic
T1 A revised model for promoter competition based on multi-way chromatin interactions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 612275
DO 10.1101/612275
A1 A. Marieke Oudelaar
A1 Caroline L. Harrold
A1 Lars L. P. Hanssen
A1 Jelena M. Telenius
A1 Douglas R. Higgs
A1 Jim R. Hughes
YR 2019
UL http://biorxiv.org/content/early/2019/04/17/612275.abstract
AB Specific communication between gene promoters and enhancers is critical for accurate regulation of gene expression. However, it remains unclear how specific interactions between multiple regulatory elements and genes contained within a single chromatin domain are coordinated. Recent technological advances allow for the investigation of multi-way chromatin interactions at single alleles in individual nuclei. This can provide insights into how multiple regulatory elements cooperate or compete for transcriptional activation. We have used these techniques in a mouse model in which the α-globin domain is extended to include several additional genes. This allows us to determine how the interactions of the α-globin super-enhancer are distributed between multiple promoters in a single domain. Our data show that gene promoters do not form mutually exclusive interactions with the super-enhancer, but all interact simultaneously in a single complex. These finding show that promoters within the same domain do not structurally compete for interactions with enhancers, but form a regulatory hub structure, consistent with the recent model of transcriptional activation in phase-separated nuclear condensates.