TY - JOUR T1 - Post-catalytic spliceosome structure reveals mechanism of 3'-splice site selection JF - bioRxiv DO - 10.1101/212811 SP - 212811 AU - Max E. Wilkinson AU - Sebastian M. Fica AU - Wojciech P. Galej AU - Christine M. Norman AU - Andrew J. Newman AU - Kiyoshi Nagai Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/11/01/212811.abstract N2 - Introns are removed from eukaryotic mRNA precursors by the spliceosome in two transesterification reactions – branching and exon ligation. Following branching, the 5'-exon remains paired to U5 snRNA loop 1, but the mechanism of 3'-splice site recognition during exon ligation has remained unclear. Here we present the 3.7Å cryo-EM structure of the yeast P complex spliceosome immediately after exon ligation. The 3'-splice site AG dinucleotide is recognised through non-Watson-Crick pairing with the 5'-splice site and the branch point adenosine. A conserved loop of Prp18 together with the α-finger and the RNaseH domain of Prp8 clamp the docked 3'-splice site and 3'-exon. The step 2 factors Prp18 and Slu7 and the C-terminal domain of Yju2 stabilise a conformation competent for 3'-splice site docking and exon ligation. The structure accounts for the strict conservation of the GU and AG dinucleotides of the introns and provides insight into the catalytic mechanism of exon ligation. ER -