PT - JOURNAL ARTICLE AU - Christopher Nosala AU - Scott C. Dawson TI - The ventral disc is a flexible microtubule organelle that depends on domed ultrastructure for functional attachment of <em>Giardia lamblia</em> AID - 10.1101/213421 DP - 2017 Jan 01 TA - bioRxiv PG - 213421 4099 - http://biorxiv.org/content/early/2017/11/03/213421.short 4100 - http://biorxiv.org/content/early/2017/11/03/213421.full AB - The parasite Giardia lamblia interacts with its host by directly attaching the lumen of the small intestine. Attachment is mediated by a cytoskeletal structure termed the ventral disc and proceeds in four distinct stages: skimming, seal formation, cell body contacts, and bare area contacts. The precise mechanism of disc-mediated attachment is unclear and attachment models rely heavily on whether or not the ventral disc is a dynamic structure. We sought to investigate the second stage of attachment in which a seal is formed beneath the ventral disc. Three-dimensional, live imaging of Giardia expressing specific ventral disc markers to the lateral crest, ventral groove, and disc body indicate dynamic movement in all of these regions. We observe seal formation by the lateral crest and determine that movement of the ventral groove region aids lateral crest seal formation. We also report the discovery of a new protein that is necessary for ventral disc formation and functional attachment (DAP_7268). Lastly, we observed that attachment largely depends on ventral disc ultrastructure as flattened discs display hindered attachment proficiency whether or not they retain the ability to form a seal. We propose a synthesized mechanism for attachment that includes flagellar hydrodynamic flow to help generate suction as well as disc conformational dynamics to aid in both hydrodynamic flow and the maintenance of negative pressure.