RT Journal Article
SR Electronic
T1 Smad9 is a key player of follicular selection in goose via keeping the balance of LHR transcription
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 213546
DO 10.1101/213546
A1 Daolun Yu
A1 Fanghui Chen
A1 Li Zhang
A1 Hejian Wang
A1 Jie Chen
A1 Zongmeng Zhang
A1 Jie Li
A1 Chaofeng Xing
A1 Honglin Li
A1 Jun Li
A1 Yafei Cai
YR 2017
UL http://biorxiv.org/content/early/2017/11/03/213546.abstract
AB The egg production of poultry depends on follicular development and selection. However, the mechanism of selecting the priority of hierarchical follicles is completely unknown. Smad9 is one of the important transcription factors in BMP/Smads pathway and involved in goose follicular initiation. To explore its potential role in goose follicle hierarchy determination, we first blocked Smad9 expression using BMP typeⅠreceptor inhibitor LDN–193189 both in vivo and in vitro. Unexpectedly, LDN–193189 administration could dramatically suppress Smad9 level and elevate egg production (7.08 eggs / bird, P< 0.05) of animals, and the estradiol (E2) and luteinizing hormone receptor (LHR) level were significantly increased (P< 0.05), but the progesterone (P4) and follicle stimulating hormone receptor (FSHR) mRNA remain unchanged. Surprisingly, Smad9 knockdown notably attenuated (P< 0.05) in E2, P4, FSHR and LHR level in goose granulosa cells (gGCs). Further chromatin immunoprecipitation (ChIP) assay of gGCs revealed that Smad9, served as a sensor of balance, bound to the LHR promoter regulating its transcription. These findings demonstrated that Smad9 is differentially expressed in goose follicles, and acts as a key player in controlling goose follicular selection.SUMMARY STATEMENT To study the hierarchical development mechanism of avian follicle, new strategies can be found to improve the egg production of low-yielding poultry, such as geese.