PT  - JOURNAL ARTICLE
AU  - Pengxue Li
AU  - Qiaozhi Yu
AU  - Chunmiao Xu
AU  - Xu Gu
AU  - Shilian Qi
AU  - Hong Wang
AU  - Fenglin Zhong
AU  - Shuang Wu
TI  - Endodermal differentiation is reconstructed by coordination of two parallel signaling systems derived from the stele in roots
AID  - 10.1101/210872
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 210872
4099  - http://biorxiv.org/content/early/2017/11/03/210872.short
4100  - http://biorxiv.org/content/early/2017/11/03/210872.full
AB  - The plant roots represent the exquisitely controlled cell fate map in which different cell types undergo a complete status transition from stem cell division and initial fate specification, to the terminal differentiation. The endodermis is initially specified in meristem but further differentiates to form Casparian strips (CSs), the apoplastic barrier in the mature zone for the selective transport between stele and outer tissues, and thus is regarded as plant inner skin. In the Arabidopsis thaliana root the transcription factors SHORTROOT (SHR) regulate asymmetric cell division in cortical initials to separate endodermal and cortex cell layer. In this paper, we utilized synthetic approach to examine the reconstruction of fully functional Casparian strips in plant roots. Our results revealed that SHR serves as a master regulator of a hierarchical signaling cascade that, combined with stele-derived small peptides, is sufficient to rebuild the functional CS in non-endodermal cells. This is a demonstration of the deployment of two parallel signaling systems, in which both apoplastic and symplastic communication were employed, for coordinately specifying the endodermal cell fate.