PT - JOURNAL ARTICLE AU - Xiaowen Liu AU - Thuy Vien AU - Jingjing Duan AU - Shu-Hsien Sheu AU - Paul G. DeCaen AU - David E. Clapham TI - PKD2 is an essential ion channel subunit in the primary cilium of the renal collecting duct epithelium AID - 10.1101/215814 DP - 2017 Jan 01 TA - bioRxiv PG - 215814 4099 - http://biorxiv.org/content/early/2017/11/07/215814.short 4100 - http://biorxiv.org/content/early/2017/11/07/215814.full AB - Mutations in either Pkd1 or Pkd2 result in Autosomal Dominant Polycystic Kidney Disease (ADPKD). Although PKD2 is proposed to be an ion channel subunit, recordings of PKD2 ion channels conflict in their properties. Using a new ADPKD mouse model, we observe primary cilia are abnormally long in cells associated with cysts. Using primary cultures of collecting duct epithelial cells, we show that PKD2, but not PKD1, is a required subunit for primary cilia ion channel. The ciliary PKD2 channel conducts potassium and sodium ions, but little calcium. We also demonstrate that PKD2 is not constitutively active in the plasma membrane, but PKD2 channels are functional in primary cilia and are sensitized by high cilioplasmic [Ca2+]. We introduce a novel method for measuring PKD2 channels heterologously expressed in primary cilia of HEK-293 cells, which will have utility characterizing Pkd2 variants that cause ADPKD in their native ciliary membrane.