PT  - JOURNAL ARTICLE
AU  - Diego R. Revinski
AU  - Laure-Emmanuelle Zaragosi
AU  - Camille Boutin
AU  - Sandra Ruiz-Garcia
AU  - Marie Deprez
AU  - Olivier Rosnet
AU  - Virginie Thomé
AU  - Olivier Mercey
AU  - Agnès Paquet
AU  - Nicolas Pons
AU  - Brice Marcet
AU  - Laurent Kodjabachian
AU  - Pascal Barbry
TI  - CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
AID  - 10.1101/218750
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 218750
4099  - http://biorxiv.org/content/early/2017/11/13/218750.short
4100  - http://biorxiv.org/content/early/2017/11/13/218750.full
AB  - Multiciliated cells (MCCs) harbour dozens to hundreds of motile cilia, which beat in a synchronized and directional manner, thus generating hydrodynamic forces important in animal physiology1, 2. In vertebrates, MCC differentiation critically depends on the synthesis and release of numerous centrioles by specialized structures called deuterosomes1-5. Little is known about the composition, organization and regulation of deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome-stage MCCs are characterized by the expression of many cell cycle-related genes. Among those, we further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene. We show that the CDC20B protein associates to the deuterosome, and is required for the production of centrioles and cilia in mouse and Xenopus MCCs. In Xenopus, centrioles and cilia were efficiently rescued in absence of CDC20B by over-expression of the protease Separase, linking CDC20B function to centriole release from deuterosomes, in analogy to centriole disengagement in mitotic cells. This work reveals the shaping of a new biological function, deuterosome-mediated centriole production in vertebrate MCCs, by adaptation between ancestral and recently evolved cell cycle-related molecules.