RT Journal Article
SR Electronic
T1 Crosslinking actin networks produces compressive force
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 614453
DO 10.1101/614453
A1 Rui Ma
A1 Julien Berro
YR 2019
UL http://biorxiv.org/content/early/2019/04/19/614453.abstract
AB Actin has been shown to be essential for clathrin-mediated endocytosis in yeast. However, actin polymerization alone is likely insufficient to produce enough force to deform the membrane against the huge turgor pressure of yeast cells. In this paper, we used Brownian dynamics simulations to demonstrate that crosslinking of a meshwork of non-polymerizing actin filaments is able to produce compressive forces. We show that the force can be up to thousands of piconewtons if the crosslinker has a high stiffness. The force decays over time as a result of crosslinker turnover, and is a result of converting chemical binding energy into elastic energy.