PT  - JOURNAL ARTICLE
AU  - Jiaxi Chen
AU  - Zhipeng Su
AU  - Chunlei Zhang
AU  - Fenge Li
AU  - Patrick Hwu
AU  - Zhen Wang
AU  - Yanping Wang
AU  - Yunsen Li
AU  - Jiao Tong
AU  - Chunchao Chen
AU  - Dapeng Zhou
TI  - Normal development and fertility of Fut1, Fut2, and Sec1 triple knockout mice
AID  - 10.1101/615070
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 615070
4099  - http://biorxiv.org/content/early/2019/04/21/615070.short
4100  - http://biorxiv.org/content/early/2019/04/21/615070.full
AB  - The fucose alpha(1,2) galactose structure (H antigen) is synthesized by α1,2 fucosyltransferases Fut1, Fut2 and Sec1. H antigen has been reported to be involved in cancer progression, neurite migration, synaptic plasticity, host-microbe interaction, blastocyst implantation, and the maintenance of gut microbiome. Genetic depletion of Fut1 or Fut2 only cause defects of alpha1,2 fucosylation in limited tissues because of enzyme redundancy. In this study, we generated mice with deficiencies in Fut1, Fut2, and Sec1 genes to deplete H antigen through BAC Engineering for the generation of ES Cell-Targeting construct. The homogenous triple knockout mice showed no significant decrease of viability or development. Mass spectrometry and Western blot analysis confirmed the absence of H blood group antigen in multiple organs. These results indicate normal development and fertility of mice devoid of blood group H. The fine pathophysiological alterations in these mice remain to be carefully studied, and they may serve as valuable tools to study gut microbiome and host-microbe interactions.Fut1α-2 fucosyltransferase IFut2α-2 fucosyltransferase IISec1α-2 fucosyltransferase IIIBlood group HFuc α2 Gal βBlood group AGalNAc α3 Fuc α2 Gal βBlood group BGal α3 Fuc α2 Gal βCFGconsortium of functional glycomicsLewis yFuc α2 Gal β4 (α3) GlcNAc βType I HFuc α2 Gal β3 GlcNAc βType II HFuc α2 Gal β4 GlcNAc βType III HFuc α2 Gal β3 GalNAc αType IV HFuc α2 Gal β3 GalNAc βType V HFuc α2 Gal β3 Ga βType VI HFuc α2 Gal β4 Glc β