RT Journal Article
SR Electronic
T1 A Cys2/His2 zinc finger protein acts as a repressor of gibberellins biosynthesis by regulating SD1/OsGA20ox2 in rice (Oryza sativa L.)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 616433
DO 10.1101/616433
A1 Min Duan
A1 Xiao-Juan Ke
A1 Hong-Xia Lan
A1 Xi Yuan
A1 Peng Huang
A1 En-Shun Xu
A1 Xiu-Ying Gao
A1 Ru-Qin Wang
A1 Hai-Juan Tang
A1 Hong-Sheng Zhang
A1 Ji Huang
YR 2019
UL http://biorxiv.org/content/early/2019/04/23/616433.abstract
AB Gibberellins (GAs) play important roles in the regulation of plant growth and development. The green evolution gene SD1 encoding gibberellin 20-oxidase 2 (GA20ox2) has been widely used in modern rice breeding. However, the molecular mechanism of how SD1/OsGA20ox2 expression is regulated remains unclear. Here we report a Cys2/His2 zinc finger protein ZFP207 acting as a transcriptional repressor of OsGA20ox2. ZFP207 was mainly accumulated in young tissues and more specifically in culm nodes. ZFP207-overexpression (ZFP207OE) plants displayed semi-dwarfism phenotype and small grains by modulating cell length. RNA interference of ZFP207 caused higher plant and longer grains. The endogenous bioactive GA levels were significantly reduced in ZFP207OE plants and application of exogenous GA3 rescued the semi-dwarf phenotype. The in vivo and in vitro studies showed that ZFP207 repressed the expression of OsGA20ox2 via binding to its promoter region. Together, ZFP207 acts as a transcriptional repressor of gibberellins biosynthesis and it may play a critical role in plant growth and development through fine-tuning GA biosynthesis in rice.