@article {SoRelle220954, author = {Elliott D. SoRelle and Derek Yecies and Orly Liba and F. Chris Bennett and Claus Moritz Graef and Rebecca Dutta and Siddhartha S. Mitra and Lydia-Marie Joubert and Samuel H. Cheshier and Gerald A. Grant and Adam de la Zerda}, title = {Wide-field dynamic monitoring of immune cell trafficking in murine models of glioblastoma}, elocation-id = {220954}, year = {2017}, doi = {10.1101/220954}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Leukocyte populations, especially tumor-associated macrophages (TAMs), are capable of mediating both anti- and pro-tumor processes and play significant roles in the tumor microenvironment. Moreover, TAMs have been shown to exert substantial influence on the efficacy of various cancer immunotherapy treatment strategies. Laboratory investigation into the behavior of TAMs has been limited by a lack of methods capable of resolving the in vivo distribution and dynamics of this cell population across wide fields of view. Recent studies have employed magnetic resonance imaging and intravital microscopy in conjunction with nanoparticle labeling methods to detect TAMs and observe their responses to therapeutic agents. Here we describe a novel method to enable high-resolution, wide-field, longitudinal imaging of leukocytes based on contrast-enhanced Speckle-Modulating Optical Coherence Tomography (SM-OCT), which substantially reduces imaging noise. We were able to specifically label TAMs and activated microglia in vivo with large gold nanorod contrast agents (LGNRs) in an orthotopic murine glioblastoma model. After labeling, we demonstrated near real-time tracking of leukocyte migration and distribution within the tumors. The intrinsic resolution, imaging depth, and sensitivity of this method may facilitate detailed studies of the fundamental behaviors of TAMs in vivo, including their intratumoral distribution heterogeneity and the roles they play in modulating cancer proliferation. In future studies, the method described herein may also provide the necessary means to characterize TAM responses to immunotherapeutic regimens in a range of solid tumors.}, URL = {https://www.biorxiv.org/content/early/2017/11/17/220954}, eprint = {https://www.biorxiv.org/content/early/2017/11/17/220954.full.pdf}, journal = {bioRxiv} }