RT Journal Article
SR Electronic
T1 Topography and refractometry of sperm cells using SLIM
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 222000
DO 10.1101/222000
A1 Liu, Lina
A1 Kandel, Mikhail E.
A1 Rubessa, Marcello
A1 Schreiber, Sierra
A1 Wheeler, Mathew
A1 Popescu, Gabriel
YR 2017
UL http://biorxiv.org/content/early/2017/11/19/222000.abstract
AB Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. Here, we employ spatial light interference microscopy (SLIM) to decouple the thickness and refractive index information of individual cells. This procedure was enabled by quantitative phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.