PT  - JOURNAL ARTICLE
AU  - Xavier Robin
AU  - Franziska Voellmy
AU  - Jesper Ferkinghoff-Borg
AU  - Conor Howard
AU  - Tom Altenburg
AU  - Mathias Engel
AU  - Craig D. Simpson
AU  - Gaye Saginc
AU  - Simon Koplev
AU  - Edda Klipp
AU  - James Longden
AU  - Rune Linding
TI  - Probability-based detection of phosphoproteomic uncertainty reveals rare signaling events driven by oncogenic kinase gene fusion
AID  - 10.1101/621961
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 621961
4099  - http://biorxiv.org/content/early/2019/04/29/621961.short
4100  - http://biorxiv.org/content/early/2019/04/29/621961.full
AB  - We describe a novel Bayesian method for estimating protein concentration and phosphorylation site occupancy ratios from mass spectrometry experiments. Our variance model assigns standard deviations to all quantitative ratios, even when only a single peptide is observed, increasing the number of quantifiable observations in a sample compared to conventional methods. We further demonstrate the application of this method using a dataset investigating the impact of the PRKAR1A-RET gene fusion in immortalized thyroid cells.