RT Journal Article
SR Electronic
T1 Genetic Single Neuron Anatomy reveals fine granularity of cortical interneuron subtypes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 219485
DO 10.1101/219485
A1 Xiaojun Wang
A1 Jason Tucciarone
A1 Siqi Jiang
A1 Fangfang Yin
A1 Bor-shuen Wang
A1 Dingkang Wang
A1 Yao Jia
A1 Xueyan Jia
A1 Yuxin Li
A1 Tao Yang
A1 Zhengchao Xu
A1 Masood A. Akram
A1 Yusu Wang
A1 Shaoqun Zeng
A1 Giorgio A. Ascoli
A1 Partha Mitra
A1 Hui Gong
A1 Qingming Luo
A1 Z. Josh Huang
YR 2017
UL http://biorxiv.org/content/early/2017/11/20/219485.abstract
AB Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of discrete cell groups from population continuum. Combining genetic labeling with high-resolution, large volume light microscopy, we established a platform of genetic single neuron anatomy that resolves, registers and quantifies complete neuron morphologies in the mouse brain. We discovered that cortical axo-axonic cells (AACs), a cardinal GABAergic interneuron type that controls pyramidal neuron (PyN) spiking at axon initial segment, consist of multiple subtypes distinguished by laminar position, dendritic and axonal arborization patterns. Whereas the laminar arrangements of AAC dendrites reflect differential recruitment by input streams, the laminar distribution and local geometry of AAC axons enable differential innervation of PyN ensembles. Therefore, interneuron types likely consist of fine-grained subtypes with distinct input-output connectivity patterns.