PT  - JOURNAL ARTICLE
AU  - Gao, Liang
TI  - A method to keep the excitation light sheet in focus in selective plane illumination microscopy
AID  - 10.1101/222497
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 222497
4099  - http://biorxiv.org/content/early/2017/11/20/222497.short
4100  - http://biorxiv.org/content/early/2017/11/20/222497.full
AB  - Keeping the excitation light sheet in focus is critical in selective plane illumination microscopy (SPIM) to ensure its 3D imaging ability. Unfortunately, an effective method that can be used in SPIM on general biological specimens to find the axial position of the excitation light sheet and keep it in focus is barely available. Here, we present a method to solve the problem. We investigate its mechanism and demonstrate its performance on a lattice light sheet microscope.