RT Journal Article
SR Electronic
T1 A method to keep the excitation light sheet in focus in selective plane illumination microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 222497
DO 10.1101/222497
A1 Gao, Liang
YR 2017
UL http://biorxiv.org/content/early/2017/11/20/222497.abstract
AB Keeping the excitation light sheet in focus is critical in selective plane illumination microscopy (SPIM) to ensure its 3D imaging ability. Unfortunately, an effective method that can be used in SPIM on general biological specimens to find the axial position of the excitation light sheet and keep it in focus is barely available. Here, we present a method to solve the problem. We investigate its mechanism and demonstrate its performance on a lattice light sheet microscope.