PT - JOURNAL ARTICLE AU - Mike Notohamiprodjo AU - Martin Goepfert AU - Susanne Will AU - Roberto Lorbeer AU - Fritz Schick AU - Wolfgang Rathmann AU - Petros Martirosian AU - Annette Peters AU - Katharina Müller-Peltzer AU - Andreas Helck AU - Susanne Rospleszcz AU - Fabian Bamberg TI - Renal and Renal Sinus Fat Volumes as Quantified by Magnetic Resonance Imaging in Subjects with Prediabetes, Diabetes, and Normal Glucose Tolerance AID - 10.1101/620146 DP - 2019 Jan 01 TA - bioRxiv PG - 620146 4099 - http://biorxiv.org/content/early/2019/04/30/620146.short 4100 - http://biorxiv.org/content/early/2019/04/30/620146.full AB - Purpose The aim of this study was to assess the volume of the respective kidney compartments with particular interest in renal sinus fat as an early biomarker and to compare the distribution between individuals with normal glucose levels and individuals with prediabetes and diabetes.Material and Methods The sample comprised N = 366 participants who were either normoglycemic (N = 230), had prediabetes (N = 87) or diabetes (N =49), as determined by Oral Glucose Tolerance Test. Other covariates were obtained by standardized measurements and interviews. Whole-body MR measurements were performed on a 3 Tesla scanner. For assessment of the kidneys, a coronal T1w dual-echo Dixon and a coronal T2w single shot fast spin echo sequence were employed. Stepwise semi-automated segmentation of the kidneys on the Dixon-sequences was based on thresholding and geometric assumptions generating volumes for the kidneys and sinus fat. Inter- and intra-reader variability were determined on a subset of 40 subjects. Associations between glycemic status and renal volumes were evaluated by linear regression models, adjusted for other potential confounding variables. Furthermore, the association of renal volumes with visceral adipose tissue was assessed by linear regression models and Pearson’s correlation coefficient.Results Renal volume, renal sinus volume and renal sinus fat increased gradually from normoglycemic controls to individuals with prediabetes to individuals with diabetes (renal volume: 280.3±64.7 ml vs 303.7±67.4 ml vs 320.6±77.7ml, respectively, p < 0.001). After adjustment for age and sex, prediabetes and diabetes were significantly associated to increased renal volume, sinus volume (e.g. βprediabetes = 10.1, 95% CI: [6.5, 13.7]; p<0.01, βDiabetes = 11.86, 95% CI: [7.2, 16.5]; p<0.01) and sinus fat (e.g. βprediabetes = 7.13, 95% CI: [4.5, 9.8]; p<0.001, βDiabetes = 7.34, 95% CI: [4.0, 10.7]; p<0.001). Associations attenuated after adjustment for additional confounders were only significant for prediabetes and sinus volume (β =4.0 95% CI [0.4, 7.6]; p<0.05). Hypertension was significantly associated with increased sinus volume (β = 3.7, 95% CI: [0.4, 6.9; p<0.05]) and absolute sinus fat volume (β = 3.0, 95%CI: [0.7, 5.2]; p<0.05). GFR and all renal volumes were significantly associated as well as urine albumin levels and renal sinus volume (β = 1.6, 95% CI: [0.2, 3.0]; p<0.05). There was a highly significant association between VAT and the absolute sinus fat volume (β = 2.75, 95% CI: [2.3, 3.2]; p<0.01).Conclusion Renal volume and particularly renal sinus fat volume already increases significantly in prediabetic subjects. There is a significant association between VAT and renal sinus fat, suggesting that there are metabolic interactions between these perivascular fat compartments.