TY - JOUR T1 - Genetic characterization of a recombinant myxoma virus leap into the Iberian hare (<em>Lepus granatensis</em>) JF - bioRxiv DO - 10.1101/624338 SP - 624338 AU - Ana Águeda-Pinto AU - Ana Lemos de Matos AU - Mário Abrantes AU - Simona Kraberger AU - Maria A. Risalde AU - Christian Gortázar AU - Grant McFadden AU - Arvind Varsani AU - Pedro J. Esteves Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/01/624338.abstract N2 - Myxomatosis is a lethal disease of wild European and domestic rabbits (Oryctolagus cuniculus) caused by a Myxoma virus (MYXV) infection, a leporipoxvirus that is found naturally in some Sylvilagus rabbit species in South America and California. The introduction of MYXV in the early 1950s into feral European rabbit populations in Australia and Europe demonstrate the best documented field example of host-virus coevolution following a cross-species transmission. Recently, a new cross-species jump of MYXV has been suggested in both Great Britain and Spain, where European brown hares (Lepus europaeus) and Iberian hares (Lepus granatensis) were found dead with lesions consistent with those observed in myxomatosis. To investigate the possibility of a new cross-species transmission event by MYXV, tissue samples collected from a wild Iberian hare found dead in Spain (Toledo region) were analyzed and deep sequenced. Our results report a new MYXV strain (MYXV Toledo) in the tissues of this species. The genome of this new strain encodes three disrupted genes (M009L, M036L and M152R) and a novel 2.8 KB recombinant region that resulted from an insertion of four novel poxviral genes towards the 5’ end of its genome. From the open reading frames inserted into the MYXV Toledo strain, a new orthologue of a poxvirus host range gene family member was identified which is related to the MYXV gene M064R. Overall, we confirmed the identity of a new MYXV strain in Iberian hares that we hypothesize was able to more effectively counteract the host defenses in hares and start an infectious process in this new host. ER -